Web7 Sep 2024 · Genetically encoded BRET sensors are often used to measure the activity of G proteins but are limited to those stimulated by wild-type receptors. Schihada et al. developed BRET sensors capable of measuring the activity of GPCRs with disease-associated mutations that confer constitutive activity. Web18 Feb 2016 · This system is very versatile allowing cellular and solid phase ELISA but also homogeneous BRET based screening assays, relative affinity determinations with competition ELISA and direct Western blotting. The new Nluc protein fusion represents a “swiss army knife solution” for today and future high throughput antibody drug …
NanoBRET™ Ubiquitination Starter Kit - Promega
Web14 Feb 2024 · It is a bioluminescence resonance energy transfer (BRET) based assay that uses NanoLuc® luciferase as the BRET energy donor and HaloTag® protein labeled … WebProducts Assay-Ready eXpress ™ and Bioassay Kits. β-Arrestin eXpress and Bioassay Kits – Complete assay-ready kits with specific cell line, optimized reagents, and plates … close command window in batch file
Functional selectivity profiling of the angiotensin II type 1 receptor ...
Web15 Jul 2024 · Bradford protein assay kit 3. Microplate reader capable of dual measurement of BL and FL. 4. 485±10-nm and 530±12.5-nm filters. 2.3 Mechanical Lysis 1. Hypotonic lysis buffer: 10 mM HEPES (pH 7.90), 1.5 mM MgCl 2, and 10 mM KCl in water. WebIn this technical note, we show how a well-known cell-based BRET2 assay was transferred from a university lab to a screening facility to be utilized in a screen for Hepatitis C virus … WebThe LanthaScreen™ TR-FRET FXR Coactivator Assay Kit contains FXR-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C). Customers who viewed this item also viewed bodybuilding tank tops women