Pb7wgf2
Splet03. mar. 2024 · For colocalization, pB7WGF2 plasmids containing EGFP-CRN C-terminal construct fusions and pGWB461 plasmids containing tagRFP-SlTCP14 fusions were transformed into Agrobacterium tumefaciens strain AGL1. For BiFC, CRN and TCP constructs were transformed into pCL112 and pCL113. Transformants were grown on … SpletLOG IN. Minimum purchase order is 150,00€. Free shipping on all products. Leave this field blank. Gateway vectors Log In - pB7WGF2 Gateway Vectors Download .Gb File - pB7WGF2 Gateway Vectors Other Resources In this section, clones and vectors co-developed by the Department … For questions related to payments, MTAs, and technical issues, please contact: … The host strain of all plasmids is E. coli DB3.1 or ccdB survival cells from …
Pb7wgf2
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Splet05. jul. 2012 · pB7WGF2/GFP-AtGRXS17 was introduced into Agrobacterium strain GV3101 as described and then used for agro-infiltration as previously described (Liu et al., 2005). For heat shock treatment, at 1.5–2 days postinfiltration (DPI), the infiltrated leaves were detached from tobacco plants, kept in Petri dishes with the moistened filter paper and ... Splet03. apr. 2024 · The full AtMiro2 coding sequence was cloned using Gateway reactions (Invitrogen) into the binary destination vector PB7WGF2 creating an N-terminal GFP fusion. Using this as template, the ...
Splet21. nov. 2024 · The GFP-OsGRXS17 construct was produced by an LR clonase reaction between the entry vector and pB7WGF2 51. As a control, a modified green fluorescent … Splet05. jul. 2024 · The pB7WGF2-WRKY33, pB7WGF2-APX1 and pB7WGF2-APX2 constructs were transformed into Agrobacterium tumefaciens strain GV3105 via electroporation. …
Splet05. jul. 2012 · pB7WGF2/GFP-AtGRXS17 was introduced into Agrobacterium strain GV3101 as described and then used for agro-infiltration as previously described (Liu et al., 2005). … Splet23. nov. 2024 · Agrobacterium tumefaciens GV3101 transformed with pB7WGF2.0 or pK7WGR2.0 harboring each gene of interest or empty (as control) were cultivated overnight at 28 °C in Luria–Bertani medium ...
Splet19. jun. 2024 · Agrobacterium tumefaciens containing overexpression vector pB7WGF2 was used to transform leaf discs of N. benthamiana. Positive lines were first screened on …
http://www.biovector.net/product/1684236.html strachan\u0027s ice cream palm harborSplet26. sep. 2024 · The construct 35Sp-GFP (pB7WGF2 empty vector) was used as a control. These constructs were introduced into Col-0 or jaz4-1 plants according to the floral-dip … strachan west of england clothSplet1.构建10kb大小，具有绿色荧光蛋白的质粒载体pb7wgf2.0； 2.用qiagen公司生产的qiafilter tm plasmidmidikit试剂盒制备质粒dna；设置质粒dna浓度为600ng/μl； 3.用sigma-aldrich … strach berounSplet28. jan. 2024 · Effectors were cloned into pB7WGF2 and the constructs were transformed into Agrobacterium tumefaciens strain GV3101. Agrobacterium cells were harvested by … strache abgasrohrSpletThe primer sequences listed on the left are provided for your reference. Addgene does not distribute primers. For sequencing plasmids in our repository, we've chosen primers … stracharSplet26. sep. 2024 · The construct 35Sp-GFP (pB7WGF2 empty vector) was used as a control. These constructs were introduced into Col-0 or jaz4-1 plants according to the floral-dip method for plant transformation (Clough and Bent, 1998). Overexpresser lines were selected by spraying a 0.0114% glufosinate ammonium (BASTA, Bayer®, Whippany, NJ, … rothman group orthopedicsSpletFull Sequences from Depositor (1) Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies … rothman hammonton nj